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Notwithstanding the variety of procedures that have been launched to beat artefacts related to details compositionality, they have not been adopted for your human microbiome study. Of note, Not one of the medical trials printed during the earlier year (besides the ones particularly addressing the compositionality dilemma [five, six]) used quantitative microbiome profiling. Below, we current how quantitative PCR (qPCR)-centered bacterial enumeration can be built-in to NGS pipelines to supply a feasible method of estimate complete abundances from NGS facts, and therefore market the use of quantitative microbiome profiling in the sector of human microbiome.

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Importantly, qPCR-based quantitative microbiome profiling enjoys the subsequent conceptual and simple Added benefits around other approaches:

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